Genetic divergence based on mtDNA COI of Eothenomys miletus from Yunnan Province

Zhu Wan-long, Mu Yuan, Cheng Jin-long, Cai Jin-hong

Abstract


In the present study, 1323 bp valid sequence of COI was obtained in Eothenomys miletus. 1323bp COI sequences, the percent of A、T、G and C was 28.1%、27.1%、27.9% and 16.9%, respectively. 118 variation sites were detected, of which including 94 parsimony-informative sites and 24 singleton sites. There were 25 transitons and 3 transversion, Ts/Tv was 8.33. We found 26 haplotypes from 80 individuals, Hd was 0.929, the average genetic distance among 26 haplotypes was 0.0209. Average Pi was 0.02192. The discrepancy of nucleotide divergence (Dxy)、level of differentiation (Fst)、net genetic distance (Da)、coefficient of differentiation (Gst) between Hengduan Mountain populations and Kunming population was very remarkable, whereas the discrepancy was unconspicuous within Hengduan Mountain populations. Phylogenetic tree of different populations based on neighbor-joining method / UPGMA method and kimura-2-paramater genetic distance showed that it contained 2 main parts. The distribution curves of the mismatch analysis on different populations may be the multimodal curves that encovered populations without expansion. Furthermore, Tajima's D、Fu and Li's D and Fu's Fs test for population showed that E. miletus didn’t go through the event of population expansion.

 

Keywords: Eothenomys miletus; Genetic divergence; Cytochrome c oxidase I


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