Effect of Herbal Extracts Supplemented diets on Non Specific Immunity and Resistance to Aeromonas hydrophila in Indian cat fish (Mystus montanus)

G. Chelladurai, T. Veni, J. Mohanraj, R. Nagarajan

Abstract


Immunostimulatory can be used for fish disease prevention as it can enhance fish resistance by increasing the nonspecific defense mechanisms. The present study was conducted to evaluate the immune stimulatory and growth promoting effects of four medicinal plants extracts using ethanol on non- specific immune response and disease resistance to A. hydrophila challenge with cat fish M. montanus. The ethanol extract of eight medicinal plants (Allium cepa, Allium sativum, Zingiber officinale, Urtica dioica, Alove vera, Azadirachta indica, Cinnamomum verum and Origanum vulgare) were screened for their antimicrobial activity against A.hydrophila. Among the eight plants tested four of them are showed good antibacterial activity because of that it has been taken for growth promoting effects of M. montanus. The four plants extracts such as Onion (Allium cepa), Garlic (Allium sativum), Ginger (Zingiber officinale), Neetle (Urti cadioica) mixed with the artificial feeds at concentration of 000(E1), 250(E2), 500(E3), 750(E4), 1000(E5) mg kg-1 of dry diet and fed to healthy fish. After 60 days of feeding a challenge trial was conducted by injection of A.hydrophila, after challenged the hematological parameters of fish were studied. The present study indicate the increase in the number of RBC, WBC, Thromobocytes, Haemoglobin, PCV, MCV, MCH and MCHC. All experimental groups showed higher survival rate compared to control at level of (P<0.05). The survival percentage was found highest in the group of E2 and lowest in control group. This result reflects that effect of herbal extracts supplemented dites on non specific immunity and resistance to A. hydrophila in Indian cat fish (Mystus montanus).

Keywords: Aeromonas hydrophila, Mystus montanus, herbal extracts, hematological parameters,


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